高低剩余采食量北京鸭品系皮脂蛋白质组差异研究

doi:10.11843/j.issn.0366-6964.2018.12.008

Abstract

Abstract:

The study aimed to compare and analyze the sebum protein components of high and low residual feed intake (RFI) Pekin duck strains, and to reveal the molecular mechanism of fat deposition differences among different RFI strains. In this paper, the sebum of 12 Pekin ducks in high RFI line and 12 Pekin ducks in low RFI line of 6 weeks old were selected as experimental material, then the protein components in sebum of different RFI strains were analyzed by using the iTRAQ proteomics and bioinformatics mathods. The results showed that a total of 859 proteins were identified, 25 proteins were highly expressed in high RFI strain, and 34 proteins were highly expressed in low RFI strain; GO analysis showed that differentially expressed proteins were mainly involved in organic acid metabolism, lipid response, lipid synthesis, and so on; Protein network interaction analysis found that the number of differentially expressed proteins involved in the lipid metabolism process was the highest; The expression of 3 genes coding differentially expressed proteins were analyzed by qRT-PCR, and the results were consistent with the corresponding proteins expression. The results showed that fatty acid synthase, lipid droplet protein, apolipoprotein and fatty acid binding protein were highly expressed in the sebum of ducks in high RFI strain, which improved the fat deposition ability of Pekin ducks in high RFI strain. The results of this experiment lay the foundation for further study of the molecular mechanism of differences in sebum of ducks of different RFI strains.

CLC Number:

S834.2

ZHANG Yun-sheng, YANG Yu-ze, GUO Zhan-bao, XIE Ming, CHANG Zhuo, ZHANG Zhi-ying, HOU Shui-sheng. Study on Differential Expression of Proteome in Sebum between High and Low Residual Feed Intake Strains of Pekin Ducks[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(12): 2593-2601.

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Study on Differential Expression of Proteome in Sebum between High and Low Residual Feed Intake Strains of Pekin Ducks

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